MCF-7

1.Cell Name

MCF-7,Human breast cancer cells

2.Cell morphology(100X)

3. Electroporation results

Transfection rate 81.0%, survival rate 72.0%

4. Electroporation parameters (No.EBCC-15001)

180V，100μs，6,1s

5. Culture Conditions

5.1 Culture medium

DMEM+10% FBS

5.2 Cryopreservation medium

95%  DMEM+5%DMSO

5.3 Cell passaging

5.3.1 Do not blow or shake the culture flask during the digestion process to prevent cell agglomeration

5.3.2 The digestion process should be carried out in a 37℃ incubator