RAW264.7

1.Cell Name

RAW264.7,Macrophages

2.Cell morphology(100X)

3. Electroporation results

Transfection efficiency 62%, survival rate 60%

4. Electroporation parameters (No.EBCC-15001)

220V，100us，6，1s

5. Culture Conditions

5.1 Culture medium

DMEM+10% FBS

5.2 Cryopreservation medium

95% complete DMEM+5%DMSO

5.3 Cell passaging

5.3.1 During the digestion process, avoid knocking or shaking the culture dish to prevent the cells from falling off

5.3.2 The digestion process should be carried out in a 37℃ incubator

5.3.3 Subculture at a ratio of 1:3, every 2 days

5.4 Tips for electroporation

5.4.1 EL buffer

5.4.2 For each electroporation, add 5μg of plasmid into 100μl electroporation volume. The concentration of plasmid extraction is 1.0-2μg/ul (A260/280=1.8-2.0). System: 1.5~5.0×10^5 cells/60μl electroporation buffer/well.