OVCAR-3

1.Cell Name

OVCAR-3, Human ovarian cancer cells

2.Cell morphology(100X)

3. Electroporation results

Transfection rate 85%, survival rate 85.3%

4. Electroporation parameters (No.EBCC-15001)

120V，100us，6，1000ms

5. Culture Conditions

5.1 Culture medium

RPMI 1640+10% FBS

5.2 Cryopreservation medium

95% RPMI 1640+ 5% DMSO

5.3 Cell passaging

5.3.1 During the digestion process, do not blow or shake the culture flask to prevent cell clumping.

5.3.2 The digestion process should be carried out in a 37°C incubator.

5.3.3 Subculture at a 1:3 ratio every other day.

5.4 Transfection techniques

It is recommended to use EK buffer for efficient transfection.