HCT15

1.Cell Name

HCT15, Human colorectal adenocarcinoma cells

2.Cell morphology(100X)

3. Electroporation results

Transfection rate 75.0%, survival rate 75.0%

4. Electroporation parameters (No.EBCC-15001)

300V, 100us, 6, 1s

5. Culture Conditions

5.1 Culture medium

RPMI 1640+ 10% FBS

5.2 Cryopreservation medium

95%RPMI 1640+5% DMSO

5.3 Cell passaging

5.3.1 During the digestion process, do not blow or shake the culture flask to prevent cell clumping.

5.3.2 The digestion process should be carried out in a 37°C incubator.

5.3.3 Passage at 1:3 every other day.

5.4 Transfection techniques

It is recommended to use EK buffer for efficient transfection