MDCK
1.Cell Name
MDCK,Canine kidney cells
2.Cell morphology(100X)
3. Electroporation results
Transfection efficiency 75%, survival rate 65%
4. Electroporation parameters (No.EBCC-15001)
200V，100μs，6,1s
5. Culture Conditions
5.1 Culture medium
DMEM+10% FBS
5.2 Cryopreservation medium
95% complete DMEM+5%DMSO
5.3 Cell passaging
5.3.1 During the digestion process, avoid knocking or shaking the culture dish to prevent the cells from falling off
5.3.2 The digestion process should be carried out in a 37℃ incubator
5.3.3 Subculture at a ratio of 1:4, once every 2 days
5.4 Tips for electroporation
5.4.1 Electrotransfer buffer: EK buffer
5.4.2 Recommended electroporation cell density and plasmid amount: For each electroporation, add 1 μg of plasmid to 100 μl of electroporation volume. The concentration of plasmid extraction is 1.0-10×10^5cell/60μl, 3-6 μg/60ul
5.4.3 Plasmid mass: 1-2 μg/ul, A260/280=1.8-2.0
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