Jurkat

1.Cell Name

Jurkat,Leukemia T lymphocytes

2.Cell morphology(100X)

3. Electroporation results

Transfection efficiency 50%, survival rate 39%

4. Electroporation parameters (No.EBCC-15001)

100V，100us，6，1s

5. Culture Conditions

5.1 Culture medium

RPMI1640+ 10% FBS

5.2 Cryopreservation medium

95% complete RPMI + 5% DMSO

5.3 Cell passaging

5.3.1 The cell seeding density is 1×10^5 cell/ml and the growth density is 1~2×10^6 cell/ml, which cannot exceed 3×10^6 cell/ml

5.3.2 Replace the volume of fresh culture medium every 2-3 days (depending on cell density)

5.4 Tips for electroporation

5.4.1 Electrotransfer buffer: EK buffer

5.4.2 Recommended electroporation cell density and plasmid volume: 3-10×10^5 cells/60μl, 3μg/60μl

5.4.3 Plasmid quality: 1-2 μg/μl, A260/A280=1.8~2.0