Hela

1.Cell Name

Hela,Human endometrial cancer cells

2.Cell morphology(100X)

3.Transfection results

Transfection rate 84.1%, survival rate 84.9%

4. Transfection conditions (No.EBCC-15001)

140v，100μs，6,1s

5. Cell Culture Conditions

5.1 Culture medium

RPMI1640 + 10% FBS

5.2 Cryopreservation conditions

95%RPMI1640+5%DMSO

5.3 Notes on Subculturing

5.3.1 During the digestion process, do not blow or shake the culture flask to prevent cell clumping.

5.3.2 Place in a 37°C incubator for digestion.

5.3.3 Subculture at a rate of 1:3 every other day.

5.4 Transfection techniques

5.4.1 Electrotransfer buffer: EK buffer

5.4.2 For each electroporation, add 1 μg of plasmid into 100 μl electroporation volume. The concentration of plasmid extraction is 1-2 μg/ul (A260/280=1.8-2.0). System: 1.5~5.0×10^5 cells/60 μl electroporation buffer/well.