HCT116

1.Cell Name

HCT116, Human colon cancer cells

2.Cell morphology(100X)

3. Electroporation results

Transfection efficiency 70%, survival rate 75%

4. Electroporation parameters (No.EBCC-15001)

300V，100s，6，1s

5. Culture Conditions

5.1 Culture medium

RPMI 1640 + 10% FBS

5.2 Cryopreservation medium

95% complete RMPI1640+5%DMSO

5.3 Cell passaging

5.3.1 During the digestion process, avoid knocking or shaking the culture dish to prevent the cells from falling off

5.3.2 The digestion process should be carried out in a 37℃ incubator

5.3.3 Subculture at a ratio of 1:3, every 2 days

5.4 Transfection techniques

5.4.1 Electroporation buffer: RMPI1640

5.4.2 For each electroporation, add 5μg of plasmid into 100μl electroporation volume. The concentration of plasmid extraction is 1.0-2μg/ul (A260/280=1.8-2.0). System: 1.5~5.0×10^5 cells/60μl electroporation buffer/well.