Caco-2

1.Cell Name

Caco-2,Human colon adenocarcinoma cells

2.Cell morphology(100X)

3. Electroporation results

Transfection rate 58.5%, survival rate 83.2%

4. Electroporation parameters (No.EBCC-15001)

120V，50us，9，1000ms

5. Culture Conditions

5.1 Culture medium

DMEM+10% FBS

5.2 Cryopreservation medium

95%  DMEM+5%DMSO

5.3 Cell passaging

5.3.1 During the digestion process, do not blow or shake the culture flask to prevent cell clumping.

5.3.2 The digestion process should be carried out in a 37°C incubator.

5.3.3 Subculture at a 1:3 ratio every other day.

5.4 Transfection techniques

Efficient transfection can be achieved using EK buffer.