A-375

1.Cell Name

A-375,Human skin malignant melanoma cells

2.Cell morphology(100X)

3. Electroporation results

Transfection rate 81.4%, survival rate 85.6%

4. Electroporation parameters (No.EBCC-15001)

120V，20μs，3，500ms

5. Culture Conditions

5.1 Culture medium

DMEM+10% FBS

5.2 Cryopreservation medium

95% DMEM + 5% DMSO

5.3 Notes on Subculturing

5.3.1 During the digestion process, do not blow or shake the culture flask to prevent cell clumping.

5.3.2 The digestion process should be carried out in a 37°C incubator.

5.3.3 Passage at 1:3 every other day.

5.4 Transfection techniques

Efficient transfection can be achieved using EK buffer.